Journal: Oncology Reports

Article Title: Inhibitory role of angiopoietin-like 4 for cancer progression in oropharyngeal squamous cell carcinoma

doi: 10.3892/or.2026.9122

Figure Lengend Snippet: Immunohistochemical staining of oropharyngeal squamous cell carcinoma. Representative cases with (A) positive and (B) negative staining of angiopoietin-like 4 are shown (lower magnification, ×100; scale bar, 250 µm; higher magnification, ×400; scale bar, 50 µm).

Article Snippet: The primers and probes were procured from Applied Biosystems (TaqMan ® Gene Expression Assays) and had the following IDs: ANGPTL4 (Hs01101127_m1), ACTB (Hs01060665_g1), MKI67 (Hs04260396_g1), BAX (Hs0018269_m1), BCL2 (Hs00608023_m1), and CASP3 (Hs00234387_m1).

Techniques: Immunohistochemical staining, Staining, Negative Staining

Journal: Oncology Reports

Article Title: Inhibitory role of angiopoietin-like 4 for cancer progression in oropharyngeal squamous cell carcinoma

doi: 10.3892/or.2026.9122

Figure Lengend Snippet: (A) Overall survival and (B) disease-free survival of patients with oropharyngeal squamous cell carcinoma based on ANGPTL4. ANGPTL4, angiopoietin-like 4.

Article Snippet: The primers and probes were procured from Applied Biosystems (TaqMan ® Gene Expression Assays) and had the following IDs: ANGPTL4 (Hs01101127_m1), ACTB (Hs01060665_g1), MKI67 (Hs04260396_g1), BAX (Hs0018269_m1), BCL2 (Hs00608023_m1), and CASP3 (Hs00234387_m1).

Techniques:

Journal: Oncology Reports

Article Title: Inhibitory role of angiopoietin-like 4 for cancer progression in oropharyngeal squamous cell carcinoma

doi: 10.3892/or.2026.9122

Figure Lengend Snippet: Fluorescence intensities of anti-angiopoietin-like 4 (red), anti-Ki-67 (green), and Hoechst (blue) for the FaDu cells. (A-C) The fluorescence intensities of the FaDu cells transfected with angiopoietin-like 4 were significantly different from those of the control. *P<0.05.

Article Snippet: The primers and probes were procured from Applied Biosystems (TaqMan ® Gene Expression Assays) and had the following IDs: ANGPTL4 (Hs01101127_m1), ACTB (Hs01060665_g1), MKI67 (Hs04260396_g1), BAX (Hs0018269_m1), BCL2 (Hs00608023_m1), and CASP3 (Hs00234387_m1).

Techniques: Fluorescence, Transfection, Control

Journal: Oncology Reports

Article Title: Inhibitory role of angiopoietin-like 4 for cancer progression in oropharyngeal squamous cell carcinoma

doi: 10.3892/or.2026.9122

Figure Lengend Snippet: CellTiter-Glo 2.0 luminescence assay revealed a significant increase in cell viability following angiopoietin-like 4 knockdown relative to the controls (set as 1.0) (P=0.010). *P<0.05.

Article Snippet: The primers and probes were procured from Applied Biosystems (TaqMan ® Gene Expression Assays) and had the following IDs: ANGPTL4 (Hs01101127_m1), ACTB (Hs01060665_g1), MKI67 (Hs04260396_g1), BAX (Hs0018269_m1), BCL2 (Hs00608023_m1), and CASP3 (Hs00234387_m1).

Techniques: Luminescence Assay, Knockdown

Journal: Oncology Reports

Article Title: Inhibitory role of angiopoietin-like 4 for cancer progression in oropharyngeal squamous cell carcinoma

doi: 10.3892/or.2026.9122

Figure Lengend Snippet: Relative gene expression levels of various mRNAs in the FaDu cells transfected with angiopoietin-like 4 small interfering RNA were determined by reverse transcription-quantitative PCR. The angiopoietin-like 4 level decreased by 38%, and MKI67 expression increased significantly. The expression of BAX decreased, and that of BCL2 increased. CASP3 expression also increased in the angiopoietin-like 4 knockdown cells.

Article Snippet: The primers and probes were procured from Applied Biosystems (TaqMan ® Gene Expression Assays) and had the following IDs: ANGPTL4 (Hs01101127_m1), ACTB (Hs01060665_g1), MKI67 (Hs04260396_g1), BAX (Hs0018269_m1), BCL2 (Hs00608023_m1), and CASP3 (Hs00234387_m1).

Techniques: Gene Expression, Transfection, Small Interfering RNA, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Knockdown

Journal: Oncology Reports

Article Title: Inhibitory role of angiopoietin-like 4 for cancer progression in oropharyngeal squamous cell carcinoma

doi: 10.3892/or.2026.9122

Figure Lengend Snippet: Correlations between ANGPTL4 expression and overall survival based on The Cancer Genome Atlas data for (A) oropharyngeal, (B) tongue and (C) laryngeal squamous cell carcinoma. The median FPKM-UQ value for ANGPTL4 was used as the cut-off to divide the groups into high and low. (D) The mean ANGPTL4 expression in head and neck squamous cell carcinoma was significantly different (P=0.042). ANGPTL4, angiopoietin-like 4.

Article Snippet: The primers and probes were procured from Applied Biosystems (TaqMan ® Gene Expression Assays) and had the following IDs: ANGPTL4 (Hs01101127_m1), ACTB (Hs01060665_g1), MKI67 (Hs04260396_g1), BAX (Hs0018269_m1), BCL2 (Hs00608023_m1), and CASP3 (Hs00234387_m1).

Techniques: Expressing

Journal: Oncology Reports

Article Title: Inhibitory role of angiopoietin-like 4 for cancer progression in oropharyngeal squamous cell carcinoma

doi: 10.3892/or.2026.9122

Figure Lengend Snippet: Significant altered gene expression in low-angiopoietin-like 4 group based on The Cancer Genome Atlas data.

Article Snippet: The primers and probes were procured from Applied Biosystems (TaqMan ® Gene Expression Assays) and had the following IDs: ANGPTL4 (Hs01101127_m1), ACTB (Hs01060665_g1), MKI67 (Hs04260396_g1), BAX (Hs0018269_m1), BCL2 (Hs00608023_m1), and CASP3 (Hs00234387_m1).

Techniques: Gene Expression

Journal: Materials Today Bio

Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions

doi: 10.1016/j.mtbio.2026.103015

Figure Lengend Snippet: CGRP could mediate endothelial cell function via macrophages. A. The representative images of tube formation assay. B. Quantitative analysis of tube formation capacity. C. Representative scratch assay images. D. The closed wound rate of scratch assay. E. GO enrichment analysis. F. Volcano plots showing DEGs with fold change > |1.5| between BA-HPCS@CGRP-treated and Control groups. Significantly upregulated genes in BA-HPCS@CGRP-treated are shown. G. The mRNA level of Angptl4 , Igf1 and Tnfsf14 . H. Angptl4 concentration in macrophage supernatants quantified by ELISA. I. The representative images of tube formation assay. J. Determination of lactate dehydrogenase (LDH) in supernatant of endothelial cell. K. Measurement of nitric oxide (NO) content in endothelial cell supernatant. M, macrophages. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance.

Article Snippet: The concentration of Angptl4 was determined via the ELISA method (CUSABIO Co., Ltd., Wuhan, China, cat# CSB-EL001712MO).

Techniques: Cell Function Assay, Tube Formation Assay, Wound Healing Assay, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Materials Today Bio

Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions

doi: 10.1016/j.mtbio.2026.103015

Figure Lengend Snippet: CGRP could mediate endothelial cell function via macrophages. A. The representative images of tube formation assay. B. Quantitative analysis of tube formation capacity. C. Representative scratch assay images. D. The closed wound rate of scratch assay. E. GO enrichment analysis. F. Volcano plots showing DEGs with fold change > |1.5| between BA-HPCS@CGRP-treated and Control groups. Significantly upregulated genes in BA-HPCS@CGRP-treated are shown. G. The mRNA level of Angptl4 , Igf1 and Tnfsf14 . H. Angptl4 concentration in macrophage supernatants quantified by ELISA. I. The representative images of tube formation assay. J. Determination of lactate dehydrogenase (LDH) in supernatant of endothelial cell. K. Measurement of nitric oxide (NO) content in endothelial cell supernatant. M, macrophages. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance.

Article Snippet: The concentration of Angptl4 was determined via the ELISA method (CUSABIO Co., Ltd., Wuhan, China, cat# CSB-EL001712MO).

Techniques: Cell Function Assay, Tube Formation Assay, Wound Healing Assay, Control, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Materials Today Bio

Article Title: Sustained-release CGRP microspheres accelerate diabetic wound healing by synergistically promoting neurovascular regeneration through modulation of macrophage and endothelial cell functions

doi: 10.1016/j.mtbio.2026.103015

Figure Lengend Snippet: In vivo evaluation of the effects of BA-HPCS@CGRP on diabetic wound healing. A. Schematic diagram of diabetic animal model preparation. B. Representative immunofluorescence staining images of CGRP and CD31 in skin tissues from normal and diabetic mice. C. Quantitative analysis of wound healing rates after treatment. D-E. Representative images and wound healing trajectories of diabetic wounds. F. H&E staining of the newly regenerated skin tissues on days 3, 7, 14, and 18. G. Masson’s trichrome staining of the newly regenerated skin tissues on days 3, 7, 14, and 18. H. The relative mRNA level of Angptl4 on day 10. ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05; ns, no significance.

Article Snippet: The concentration of Angptl4 was determined via the ELISA method (CUSABIO Co., Ltd., Wuhan, China, cat# CSB-EL001712MO).

Techniques: In Vivo, Animal Model, Immunofluorescence, Staining